ACELL October 46/4
نویسندگان
چکیده
Bahar, Sonya, Christopher T. Gunter, Cheryl Wu, Scott D. Kennedy, and Philip A. Knauf. Persistence of external chloride and DIDS binding after chemical modification of Glu-681 in human band 3. Am. J. Physiol. 277 (Cell Physiol. 46): C791–C799, 1999.—Although its primary function is monovalent anion exchange, the band 3 protein also cotransports divalent anions together with protons at low pH. The putative proton binding site, Glu-681 in human erythrocyte band 3, is conserved throughout the anion exchanger family (AE family). To determine whether or not the monovalent anion binding site is located near Glu-681, we modified this residue with Woodward’s reagent K (N-ethyl-5-phenylisoxazolium-38-sulfonate; WRK). Measurements of Cl2 binding by 35Cl-NMR show that external Cl2 binds to band 3 even when Cl2 transport is inhibited ,95% by WRK modification of Glu-681. This indicates that the external Cl2 binding site is not located near Glu-681 and thus presumably is distant from the proton binding site. DIDS inhibits Cl2 binding even when WRK is bound to Glu-681, indicating that the DIDS binding site is also distant from Glu-681. Our data suggest that the DIDS site and probably also the externally facing Cl2 transport site are located nearer to the external surface of the membrane than Glu-681.
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ACELL October 46/4
NABENDU S. CHATTERJEE,1 CHANDIRA K. KUMAR,1 ALVARO ORTIZ,1 STANLEY A. RUBIN,2 AND HAMID M. SAID1 1Medical Research Service, Veterans Affairs Medical Center, Long Beach 90822, and Department of Medicine and Physiology/Biophysics, University of California School of Medicine, Irvine 92697; and 2Veterans Affairs West Los Angeles, Los Angeles 90073, and Department of Medicine, University of Californ...
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Illek, Beate, Lei Zhang, Nancy C. Lewis, Richard B. Moss, Jian-Yun Dong, and Horst Fischer. Defective function of the cystic fibrosis-causing missense mutation G551D is recovered by genistein. Am. J. Physiol. 277 (Cell Physiol. 46): C833–C839, 1999.—The patch-clamp technique was used to investigate the effects of the isoflavone genistein on disease-causing mutations (G551D and DF508) of the cys...
متن کاملACELL October 46/4
Parfenova, Helena, John Haffner, and Charles W. Leffler. Phosphorylation-dependent stimulation of prostanoid synthesis by nigericin in cerebral endothelial cells. Am. J. Physiol. 277 (Cell Physiol. 46): C728–C738, 1999.— Nigericin decreases intracellular pH (pHi) and stimulates prostanoid (PG) synthesis in endothelial cells from cerebral microvessels of newborn pigs. Nigericin-induced PG produc...
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